Northern blotting

Methods : Total RNA was extracted and used for northern blotting .

方法：提取总RNA，进行Northern印迹杂交；

The expression of three gene in murine tissues were analyzed by Northern blotting .

应用Northern印迹杂交法研究基因在小鼠组织中的表达情况。

The expression activity of bcl-2 and c-myc genes were determined using Northern blotting .

应用Northen印迹检测bcl-2、c-myc基因表达活性。

Identification of Differential Expression ESTs in Sheep Ovarian Tissue by Reverse Northern Blotting

反向northernblotting对绵羊卵巢组织差异表达ESTs的鉴定

The expression of chloroplast Cu / Zn SOD gene was determined using northern blotting analysis .

基因表达分析表明：叶绿体Cu/ZnSOD基因在幼嫩的茎、花中有微量的表达，在叶片中高效表达；

The expression pattern of Arachin was also studied by Northern blotting and hybridization .

通过Northern印迹杂交初步研究了花生球蛋白基因的表达模式。

The length of mRNA hybridized with cDNA was detected by Northern blotting .

并用Northern杂交实验检测与cDNA杂交的mRNA长度。

Northern blotting analysis was used to check TKc gene expression in G418 resistant glioma cells .

Northern杂交检测抗G418胶质瘤细胞中的TKc基因表达。

Northern blotting demonstrated differential expression of the mRNA transcript in the two thymic stromal cell lines .

Northern杂交分析显示，该基因mRNA转录本在两种不同胸腺基质细胞中的表达存在显著差异。

The expression activity of bcl 2 , c myc and p53 genes were determined using Northern blotting .

应用Northern印迹检测bcl2、cMyc和p53基因表达活性。

The transformants were confirmed by PCR , Southern blotting , RT-PCR and Northern blotting analysis .

经PCR、Southern印迹分析、RT-PCR和Northern检测，初步证实AtNHX1基因已整合至荞麦基因组中。

Expression activity of MMP-2 and osteopontin genes was detected by Northern blotting and MMP-2 zymography analysis .

应用Northern印迹和Ⅳ型胶原酶（MMP-2）酶图分析检测MMP-2和骨桥蛋白表达的变化。

The normal cervical tissues were used as control . Northern blotting was utilized to reveal the expression profile of the novel gene .

正常宫颈组织作为对照。应用northernblotting实验观察其在人类各种正常组织中的表达情况；

There are 3 methods to detect the ob gene expression : Radioactive Immunological Assay , Western blotting and Northern blotting .

检测ob基因表达的方法有放射免疫分析法、Western杂交法和Northern杂交法三种。

Methods DNA gel electrophoresis and Northern blotting hybridization were used to detect the apoptosis and the level of c myc gene , respectively .

方法应用体外细胞培养法，DNA凝胶电泳技术、Northern印迹杂交技术。

The expression of PNP gene was identified by reverse transcriptase ( RT ) - PCR and Northern blotting .

应用逆转录（RT）鄄PCR和RNA印迹法（northernblotting）鉴定PNP基因的表达。

Trypan blue exclusion test and Northern blotting hybridization were used to detect the cell proliferation and the expression of c myc gene .

同时取作用24h的细胞利用Northern印迹杂交检测细胞中cMyc基因的表达。

Therefore , this result together with the expression of other PR genes and defense genes need to be confirmed by RT-PCR and Northern blotting .

但该结果连同其他PR蛋白编码基因及其他防卫基因的表达还需要采用RT-PCR和Northern杂交等方法进一步确认。

Reverse Northern blotting was employed to eliminate the false positive ESTs . The true positive EST were cloned and sequenced .

应用反Northern杂交去除假阳性，对真阳性的表达序列标签（EST）进行克隆、测序及同源性比较。

Results : RT-PCR and Northern blotting analysis confirmed the expression of PNP gene in BEL7402 and BEL7402 / PNP cells .

结果：RT鄄PCR和RNA印迹法证实转染的PNP基因能在BEL7402和BEL7402/PNP细胞中表达。

Methods : Using the traditional molecular biology methods ( cell synchronization , molecular hybridization & Western blotting , Northern blotting , etc. ) examine the gene expression .

方法：通过实验室常规分子生物学手段（细胞同步化、分子杂交&Northern杂交、Western杂交等）检测相关基因的表达变化。

Results The expression of S gene was confirmed by Northern blotting , Western blotting , and ELISA ( for both antigen and antibody detection ) .

结果S基因的表达已通过Northern印迹杂交、Western印迹杂交和ELISA（抗原及抗体检测）予以证实。

The levels of ghrelin mRNA were tested by Northern blotting , and the ghrelin content in gastric tissue and plasma detected by RIA .

用放射免疫法测定胃组织及血浆ghrelin水平，以northernblotting检测胃组织中ghrelin的mRNA水平。

The results of RT-PCR , Northern blotting , and Western blotting confirmed that RANTES gene had expressed successfully in transgenic A. annua plants .

RT-PCR、Northern印迹、Western印迹杂交结果证实，RANTES基因已在转基因青蒿植株中成功表达。

The c-fos probe was prepared , and the expression of c-fos gene after treatment of 0.025 % FLE was detected with the northern blotting method .

制备c-fos探针，并以Northern杂交的方法检测c-fos在0.025%FLE处理后的表达情况。

DNA extracted by the method was applicable to PCR , restriction enzyme digesting and Southern blotting , while RNA to Northern blotting and cDNA synthesis .

用该方法提取的DNA完整、纯度高，可用于PCR、限制性内切酶酶切和Southern杂交等分子生物学操作；提取RNA产量高，RNA无降解，纯度高，适用Northern和cDNA合成等分子生物学操作。

Something about the assays of PEAS gene > Southern blotting , Northern blotting and the expression of the transplants in field were to be further studied .

关于后续的PEAS基因的PCR检测、Southern杂交、Northern杂交以及转基因植株田间的表达等问题在以后的试验中将继续进行研究。

By the results of RT-PCR and Northern blotting , we found that ob gene is expressed in porcine subcutaneous fat tissues , but not in porcine liver .

根据RT一PCR和Northern杂交两方面的实验结果，证明猪ob基因在皮下脂肪组织中表达，而在肝脏组织中不表达。

The result of Northern blotting suggested that SA could induce PR-1 and PR-5 gene expression in the leaves of Yali pear locally and systemically .

Northern杂交结果表明，SA能局部和系统地诱导鸭梨叶片PR-1、PR-5基因的表达。

The differences of IL-8 mRNA expression in eutopic endometrium of patients between with and without endometriosis , and the differences among different endometriotic lesions were further studied by Northern Blotting .

Northern杂交比较不同类型异位病灶之间以及内异症患者的在位内膜与非内异症的对照子宫内膜之间IL鄄8mRNA表达强度的差异。